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Fig. 2 | Chinese Neurosurgical Journal

Fig. 2

From: Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells

Fig. 2

HOXA11-AS regulates glioma stemness in vitro. a mRNA expression of HOXA11-AS genes was determined in GBM cell lines and normal epithelial cell cultures by qRT-PCR. Expression levels are shown relative to the expression in HEK293 cells, which was used as a control and set as 1. b qRT-PCR was used to detect HOXA11-AS expression of U87MG cells with lenti-HOXA11-AS knockdown. c qPCR panels represent HOXA11-AS expression in U87MG cells infected with lentivirus-HOXA11-AS overexpression and incubated with different concentrations of doxycyclin (dox) in the culture medium for 2 days. d and e HOXA11-AS expressing or silenced U87MG cells (1 × 103) were seeded in ultra-low attachment plate and allowed to grow for 10 days in stem cell culture medium. Images were taken under light microscope and the number of spheres (size >50 μm) was counted. (**p < 0.01; ****p < 0.0001)

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