Fig. 2.

Inhibition of SPI1 in glioma cells weakened the cell growth and metastasis in vitro. a RT-qPCR analysis of SPI1 mRNA levels after the transfection of the si-NC or si-SPI1 small interfering RNAs. SPI1 expression was normalized to GAPDH, Error bars indicate mean ± SD of 3 independent experiments.**, p< 0.01; ***, p< 0.001. b Western blot analysis of SPI1 protein levels after the transfection of the si-NC or si-SPI1 small interfering RNAs. SPI1 expression was normalized to β-Actin, Error bars indicate mean ± SD of 3 independent experiments. ***, p< 0.001. c Knockdown of SPI1 inhibited cell proliferation on the basis of CCK8 assays. Error bars represent the mean ± SD of 5 independent experiments. **, p< 0.01;***, p< 0.001. d Inhibition of SPI1 decreased cell migration as determined by transwell assays. The bar chart represents the migration cell numbers. Error bars represent the mean ± SD of 5 different field. ***, p< 0.001. e Inhibition of SPI1 decreased cell migration as determined by wound healing assay. The bar chart represents the percentage of distance at 24h or 48h divided by the distance at 0h. Date are presented as mean ± SD of 3 independent experiments. ***, p< 0.001