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Table 1 Antibodies and their concentrations, manufacturers, and applications for Western blot

From: CD4 T cell deficiency attenuates ischemic stroke, inhibits oxidative stress, and enhances Akt/mTOR survival signaling pathways in mice

Antibodies Source Dilutions Manufacturer Catalog no.
iNOS Mouse 1:10,000 BD Biosciences 610431
Arginase I Goat 1:100 Santa Cruz 18354
p-PTEN (Ser380) Rabbit 1:1000 Cell Signaling 9551
p-Akt (Ser473) Rabbit 1:100/1:1000 Cell Signaling 9271
p-PRAS40 (Thr246) Rabbit 1:100/1:1000 Cell Signaling 2997
p-P70S6K Rabbit 1:1000 Cell Signaling 9205
p-S6 Rabbit 1:1000 Cell Signaling 4857
β-Actin Rabbit 1:1000 Cell Signaling 4967