Table 1 Antibodies and their concentrations, manufacturers, and applications for Western blot
Antibodies | Source | Dilutions | Manufacturer | Catalog no. |
|---|---|---|---|---|
iNOS | Mouse | 1:10,000 | BD Biosciences | 610431 |
Arginase I | Goat | 1:100 | Santa Cruz | 18354 |
p-PTEN (Ser380) | Rabbit | 1:1000 | Cell Signaling | 9551 |
p-Akt (Ser473) | Rabbit | 1:100/1:1000 | Cell Signaling | 9271 |
p-PRAS40 (Thr246) | Rabbit | 1:100/1:1000 | Cell Signaling | 2997 |
p-P70S6K | Rabbit | 1:1000 | Cell Signaling | 9205 |
p-S6 | Rabbit | 1:1000 | Cell Signaling | 4857 |
β-Actin | Rabbit | 1:1000 | Cell Signaling | 4967 |