Fig. 1.
From: Study on the role of transcription factor SPI1 in the development of glioma
Expression levels of SPI1 in glioblastoma tissues. a RT-qPCR analysis of SPI1 expression in glioblastoma tissues compared with the control brain tissues. Error bars indicate the mean ± standard deviation of three independent experiments. (N: normal brain tissue, T: glioma tissue, green line represents T/N=1). b The value of ΔCt was used to show the expression level of SPI1 (ΔCt =Ct (SPI1) − Ct (GAPDH)) in the 10 paired human glioblastoma tissues and control brain tissues (P < 0.01). c Western blot analysis of SPI1 protein expression in three pair glioblastoma tissues compared with the normal tissues. The differences between tumor group and normal group were tested by using independent-samplest-test. d Protein expression level of SPI1 was normalized to GAPDH and quantified using Image J. Error bars represent the mean ± SD of 3 different tests. ***, p < 0.001. e Expression levels of SPI1 mRNA with glioma tissues and control tissues in GEPIA database . *, p < 0.05. f Expression levels of SPI1 protein with glioma tissues and control tissues in UALCAN database. g Survival curve of low SPI1 expression group and high SPI1 expression group